Serum samples were stored at −80°C prior to ELISA. The detection range of SEMA4D was 1.56–100 ng/mL. The concentrations of human ADAM17 in serum samples were determined using DuoSet ELISA kit (R&D ...
A disintegrin and metalloproteases ADAM10 and ADAM17 have been demonstrated to be mainly responsible for this shedding, resulting in either full-length fragments or sub-fragments called KL1 and KL2.
Finally, using the example of the Alzheimer-related proteases BACE1, ADAM10 and γ-secretase, as well as ADAM17 and signal peptide peptidase like 3 (SPPL3), we illustrate how substrate identification ...
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